Effects of Drugs on the Frog Heart
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Visual - Frog Dissection
Visual - Frog Heart Isolation
Direction:Inject drug one by one and observe their effect on the frog heart.
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Experimental Pharmacology Series - Heart
Effects of Drugs on the Isolated Heart of Frog
Aim of the experiment
To study the effects of Drugs on the Isolated Heart of Frog
To study the effects of Drugs on the Isolated Heart of Frog
Overview
Many drugs act on the heart. Adrenergic and cholinergic drugs produce opposite effects. These drugs act through respective receptors. Some drugs act directly on the heart. This experiment demonstrates the effects of a few drugs (agonists, antagonists, calcium and potassium) on the isolated heart of frog.
Materials required
- Starling's heart lever
- Stand
- A kymograph with drum and smoked paper
Solutions
Volume of Following solutions to be injected is 0.2 ml
| S.No. | Name of Drug | Dose(mcg) | Concentration |
|---|---|---|---|
| 1. | Potassium Chloride | 2000 | 10 mg/ml |
| 2. | Epinephrine(Adrenaline) | 2 | 10 mcg/ml |
| 3. | Norepinephrine(Noradrenaline) | 2 | 10 mcg/ml |
| 4. | Isoprenaline | 2 | 10 mcg/ml |
| 5. | Propranolol | 200 | 1 mg/ml |
| 6. | Acetylcholine | 2 | 10 mg/ml |
| 8. | Calcium Chloride | 2000 | 10 mg/ml |
| 9. | Atropine sulphate | 20 | 100 mg/ml |
Volume of above solutions to be injected = 0.2 ml; mcg = micrograms
PROCEDURE :
1. Isolation of frog heart is done as per the routine procedure.
2. Inject the drugs and observe the following parameters
(a) a. Heart rate
(b) b. Tone
(c) c. Force of contraction
3 Tabulate the data.
Choose a Drug and Inject
Answer the following questions
1. List cardiac stimulants and depressants ?
2. Why should I.V. Potassium and Calcium injections be given slowely ?
3. Why is the dopamine preffered over adrenaline in cardiogenic shock ?
4. List the uses and adverse effect of (i) Propranolol (ii) Digoxin (iii) Adrenaline ?
5. List cardiac depressants ?
Effects of Drugs on the Frog Heart
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Experimental Pharmacology Software
Observation Table
| Sr. No. | Drug/Chemical | Dose (in mcg) | Basal Heart Rate | Heart rate (Beats/min) | Amplitude(Height-Increased-Decreased) |
|---|---|---|---|---|---|
| 1 | |||||
| 2 | |||||
| 3 | |||||
| 4 | |||||
| 5 | |||||
| 6 | |||||
| 7 | |||||
| 8 |
Result: Different drugs have shown response as per their pharmacological receptor-dependent action.
Result
Bureau For Health And Education Status Upliftment (India)
Experimental Pharmacology Software
Result Table
| Sr. No. | Drug/Chemical | Dose (in mcg) | Basal Heart Rate | Heart rate (Beats/min) | Amplitude(Height-Increased-Decreased) |
|---|---|---|---|---|---|
| 1 | Epinephrine (Adrenaline) | 2 | 20 | 36 | Increasead |
| 2 | Norepinephrine (Noradrenaline) | 2 | 25 | 20 | Increased |
| 3 | Isoprenaline | 2 | 26 | 40 | Increased |
| 4 | Propranolol | 200 | 25 | 17 | Decreased |
| 5 | Potassium Chloride | 2000 | 25 | 01 | Decreased |
| 6 | Acetylcholine | 2 | 26 | 15 | Deacreased |
| 7 | Calcium Chloride | 2000 | 25 | 01 | Increased |
| 8 | Atropine Sulphate | 20 | 25 | 30 | No Major effect |
Result: Different drugs shown response as per their pharmacological receptors dependent action. Results of effect of each drug is tabulated above.